ABOUT ijhrs


International Journal of Health and Rehabilitation Sciences

International Journal of Health and Rehabilitation Sciences
ISSN (online) : 2278-9030
ISSN (Print) : 2278-9014
ISSUES : 4/year
PUBLISHER : Rehabilitation Without Borders

ARTICLE TOOLS


Original Research 


Detection of mecA genes of Methicillin-Resistant Staphylococcus aureus by Polymerase Chain Reaction

Sajith Khan AK, Preetha J Shetty, Lakshmi Sarayu Y, Anandi Chidambaram, Ramesh Ranganathan.

Cited by (2)

Abstract
Background: Methicillin-Resistant Staphylococcus aureus (MRSA) infections have become a global health problem particularly in hospital setup causing simple skin infections to life-threatening infections. In many developing countries, including India, the situation appears gloomy due to inadequate or poor implementation of policy on infection control, lack of political will, inadequate resources including shortage of skilled manpower, poor motivation of health care workers and researchers.

Objective: The present study describes a rapid and accurate PCR for detection of clinically relevant antibiotic resistance gene of Staphylococcus aureus.

Materials and Methods: A total of 586 Staphylococcus positive clinical samples were collected under aseptic precautions from patients attending various departments in a tertiary care hospital in south India. Staphylococcus aureus isolates were identified based on cultural characteristics, biochemical reactions and positive tube coagulase test. Methicillin resistance was determined by Kirby-Bauer’s disc diffusion method. The PCR was used for mecA gene detection from MRSA strains.

Results: Of the total 586 Staphylococcal aureus isolated, 236 (40.2%) strains were MRSA. Thirty-five MSRA strains were randomly selected for PCR assay. Thirty three MRSA strains (94%) were mecA gene positive and two MRSA strains were mecA negative visualised on 2% agarose gel electrophoresis.

Conclusion: The PCR assay was found to be rapid and accurate procedure for the detection of MRSA strains as compared to the conventional methods since the reporting time is less and can help efficiently in infection management.

Key words: MRSA, PCR, mecA genes, Staphylococcal aureus


 
ARTICLE TOOLS
Abstract
PDF Fulltext
How to cite this articleHow to cite this article
Citation Tools
Related Records
 Articles by Sajith Khan AK
Articles by Preetha J Shetty
Articles by Lakshmi Sarayu Y
Articles by Anandi Chidambaram
Articles by Ramesh Ranganathan
on Google
on Google Scholar


REFERENCES
1. Chambers HF. The changing epidemiology of Staphylococcus aureus. Emerging Infect Dis 2001; 7: 178-182. [DOI via Crossref]    [Pubmed]    [PMC Free Fulltext]   
2. Shopsin B, Kreiswrith B. Molecular epidemiology of Methicillin-resistant Staphylococcus aureus. Emerg Infect Dis 2001; 7: 322-326. [DOI via Crossref]   
3. Sachdev D, Amladi S, Nataraj G, Baveja S, Kharkar V, Maharajan, Khopkar U. An Outbreak of Methicillin-resistant Staphylococus aureus (MRSA) infection in dermatology indoor patients. Indian J Dermatol. Venereol. Leprol. 2003; 69:377- 380. [Pubmed]   
4. Andre Z, Carlos C, Thierry V. Penicillin-binding proteins and b-lactamresistance. FEMS Microbiol Rev. 2008; 32: 361-385. [DOI via Crossref]    [Pubmed]   
5. Matsuhashi M, Song MD, Ishino F, Wachi M, Doi M, Inoue M, Ubukata K, Uamashita N, Konno M. Molecular cloning of the gene of a penicillin-binding protein supposed to cause high resistance to beta-lactam antibiotics in Staphylococcus aureus. J. Bacteriol. 1986; 167:975-980. [Pubmed]    [PMC Free Fulltext]   
6. Katayama Y, Ito T, Hiramatsu K. A new class of genetic element, Staphylococcus cassette chromosome mec, encodes methicillin resistance in Staphylococcus aureus. Antimicrob. Agents Chemother. 2000; 44:1549-1555. [DOI via Crossref]   
7. Ito T, Katayama Y, Asada K, Mori N, Tsutsumimoto K, Tiensasitorn C, Hiramatsu K. Structural comparison of three types of Staphylococcal Cassette Chromosome mec integrated in the Chromosome in methicillin resistant Staphylococcus aureus. Antimicrob. Agents Chemother. 2001. 45:1323-1336. [DOI via Crossref]    [Pubmed]    [PMC Free Fulltext]   
8. Jonas D, Speck M, Daschner FD, Grundmann H. Rapid PCR based identification of MRSA from screening swabs. J Clin Microbiol. 2002; 40 (5): 1821-1823. [DOI via Crossref]    [Pubmed]    [PMC Free Fulltext]   
9. Mehrotra M, Wang G, Johnson WM. Multiplex PCR for detection of genes for Staphylococcus aureus enterotoxins, exfoliative toxins, toxic shock syndrome toxin 1, and methicillin resistance. J. Clin. Microbiol. 2000; 38: 1032-1035. [Pubmed]    [PMC Free Fulltext]   
10. Gernald J Collee. Andrew G Fraser, Barrie P. Marmion, Anthony Simmons. Mackie and McCarthey's book on practical Medical Microbiology. 1996; 14 :255-256
11. National committee for Clinical Laboratory Standards. Performance standards of antimicrobial susceptibility testing, 11th informational supplement. 2001; 21(1).
12. Arakere G, Nadig S, Swedberg G, Macaden, Amarnath SK, Raghunath D. Genotyping of methicillin-resistant Staphylococcus aureus strains from two hospitals in Bangalore, South India. J. Clin. Microbiol., 2005; 43: 3198-3202. [DOI via Crossref]    [Pubmed]    [PMC Free Fulltext]   
13. Oliveira DC, de Lencastre H. Multiplex PCR strategy for rapid identification of structural types and variants of the mec Element in methicillin- resistant Staphylococci aureus. Antimicrob. Agents Chemother. 2002; 46 : 2155 – 2161 [DOI via Crossref]    [PMC Free Fulltext]   
14. Murakami K, Minamide W, Wada K, Nakamura E, Teraoka H, Watanabe S. Identification of Methicillin-resistant Strains of Staphylococci by polymerase chain reaction. J Clin Microbiol. 1991; 29: 2240-2244. [Pubmed]    [PMC Free Fulltext]   
15. Sabath LD. Mechanisms of resistance to beta lactumantibitics in strains of Staphylococcus aureus. Ann. Intern. Med. 1982; 97: 339-344. [DOI via Crossref]    [Pubmed]   
16. Kalhor H, Sharuati L, Validi M, Tabatabaiefar MA, Nafisi MR. Comparison of Agar Screen and duplex-PCR methods in determination of methicillin-resistant Staphylococcus aureus (MRSA) strains isolated from nasal carriage. African Journal of Microbiology Research. 2012; 6(16): 3722-3726.
17. Vidhani S, Mehndiratta PL, Mathur MD. Study of Methicillin resistant Staphylococcus aurues isolates from high risk patients. Indian J Med Microbial. 2001; 19 (2) : 87-90.
18. Anupurba S, Sen MR, Nath G, Sharma BM, Gulati AK, Mohapatra TM. Prevalence of Methicillin Resistant Staphylococcus aureus in a Tertiary Referral Hospital in Eastern Uttar Pradesh. Indian Journal of Medical Microbiology. 2003; 21 (1): 49-51. [Pubmed]   
19. Hanumanthappa AR, Chandrappa NR, Rajasekharappa MG. Prevalance of Methicillin resistant Staphylococcus aureus in Karnataka. Indian J Pathol.Microbiol, 2003; 46 (1) : 129-132. [Pubmed]   
20. Al Salihy SM, James AM. Loss of methicillin resistance from resistant strains of Staphylococcus aureus. Lancet. 1972; 331-332. [DOI via Crossref]   
21. Hiramatsu K, Suzuki E, Takayama H, Katayama Y, Yokota T. Role of penicillinase plasmids in the stability of the mecA gene in methicillin – resistant Staphylococcus aureus. Antimicrob. Agents. Chemother. 1990; 34: 600-604. [DOI via Crossref]    [Pubmed]    [PMC Free Fulltext]   

This Article Cited By the following articles

Farklı Klinik Örneklerden İzole Edilen Staphylococcus aureus İzolatlarında Metisilin, Yüksek Düzey Mupirosin ve Fusidik Asit Direncinin Fenotipik ve Genotipik Olarak Araştırılması
2017; 7(3): 131.

1
 
Nursing Students’ Understanding of the Concept of Self-Esteem: a Qualitative Study
J Caring Sci 2016; 5(1): 33.

2
 
How to Cite this Article
Pubmed Style

Sajith Khan AK, Preetha J Shetty, Lakshmi Sarayu Y, Anandi Chidambaram, Ramesh Ranganathan. Detection of mecA genes of Methicillin-Resistant Staphylococcus aureus by Polymerase Chain Reaction. IJHRS. 2012; 1(2): 64-68. doi:10.5455/ijhrs.000000011


Web Style

Sajith Khan AK, Preetha J Shetty, Lakshmi Sarayu Y, Anandi Chidambaram, Ramesh Ranganathan. Detection of mecA genes of Methicillin-Resistant Staphylococcus aureus by Polymerase Chain Reaction. http://www.ijhrs.org/?mno=28517 [Access: September 26, 2021]. doi:10.5455/ijhrs.000000011


AMA (American Medical Association) Style

Sajith Khan AK, Preetha J Shetty, Lakshmi Sarayu Y, Anandi Chidambaram, Ramesh Ranganathan. Detection of mecA genes of Methicillin-Resistant Staphylococcus aureus by Polymerase Chain Reaction. IJHRS. 2012; 1(2): 64-68. doi:10.5455/ijhrs.000000011



Vancouver/ICMJE Style

Sajith Khan AK, Preetha J Shetty, Lakshmi Sarayu Y, Anandi Chidambaram, Ramesh Ranganathan. Detection of mecA genes of Methicillin-Resistant Staphylococcus aureus by Polymerase Chain Reaction. IJHRS. (2012), [cited September 26, 2021]; 1(2): 64-68. doi:10.5455/ijhrs.000000011



Harvard Style

Sajith Khan AK, Preetha J Shetty, Lakshmi Sarayu Y, Anandi Chidambaram, Ramesh Ranganathan (2012) Detection of mecA genes of Methicillin-Resistant Staphylococcus aureus by Polymerase Chain Reaction. IJHRS, 1 (2), 64-68. doi:10.5455/ijhrs.000000011



Turabian Style

Sajith Khan AK, Preetha J Shetty, Lakshmi Sarayu Y, Anandi Chidambaram, Ramesh Ranganathan. 2012. Detection of mecA genes of Methicillin-Resistant Staphylococcus aureus by Polymerase Chain Reaction. International Journal of Health and Rehabilitation Sciences (IJHRS), 1 (2), 64-68. doi:10.5455/ijhrs.000000011



Chicago Style

Sajith Khan AK, Preetha J Shetty, Lakshmi Sarayu Y, Anandi Chidambaram, Ramesh Ranganathan. "Detection of mecA genes of Methicillin-Resistant Staphylococcus aureus by Polymerase Chain Reaction." International Journal of Health and Rehabilitation Sciences (IJHRS) 1 (2012), 64-68. doi:10.5455/ijhrs.000000011



MLA (The Modern Language Association) Style

Sajith Khan AK, Preetha J Shetty, Lakshmi Sarayu Y, Anandi Chidambaram, Ramesh Ranganathan. "Detection of mecA genes of Methicillin-Resistant Staphylococcus aureus by Polymerase Chain Reaction." International Journal of Health and Rehabilitation Sciences (IJHRS) 1.2 (2012), 64-68. Print. doi:10.5455/ijhrs.000000011



APA (American Psychological Association) Style

Sajith Khan AK, Preetha J Shetty, Lakshmi Sarayu Y, Anandi Chidambaram, Ramesh Ranganathan (2012) Detection of mecA genes of Methicillin-Resistant Staphylococcus aureus by Polymerase Chain Reaction. International Journal of Health and Rehabilitation Sciences (IJHRS), 1 (2), 64-68. doi:10.5455/ijhrs.000000011



Online Submission & Review

Abstracted & Indexed in:

 

  • Budapest Open Access Initiative (BOAI)

  • Directory of Open Access Journals (DOAJ)

  • Directory of Science 

  • GetCITED

  • Google Scholar

  • ICMJE

  • Index Copernicus International (7.43)

  • Index Scholar

  • J - Gate

  • Journal Index

  • Journal TOCs (UK)

  • ResearchBib

  • Scirus (Elsevier)

  • Scopemed

  • Ulrichsweb Global Serials Directory (ProQuest)

  • WHO (HINARI)